1	Impact of Acoustic Non-Contact Transfer Upon Compound Management and Ultra High Throughput Screening
2	Automation at Bristol-Myers Squibb
3	Current Platforms CRS Rail systems All compatible for 384 and low volume (LV) 384 well plates Three systems 1536 well plate enabled Polara scheduler operated Peripheral equipment is virtually plug and play
4	384 and 1536-well Screening 384-well HTS is standard with some automated 1536-well screening
5	HTS Vs UHTS WHY? UHTS = >100K per screen/per system/per day
6	Fully Automated UHTS Proof of Principal Miniaturization works- Assay performance is similar to Std Vol 384 Pharmacology matches lower density plates Current data from 1536-well FP assay at BMS
7	The Past to the Future
8	Flexible UHTS Bringing it all together
9	Automated Systems at BMS
10	Echo 550
11	Drop Formation
12	Droplet Ejection
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14	1536-well Print
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16	Acoustic Droplet Ejection Benefits Compound conservation Disparate fill volumes acceptable Auditing Water content in DMSO via well auditing Dropped well information Cherry Picking
17	Acoustic Droplet Ejection Cost Savings
18	Acoustic Droplet Ejection Cost Savings-Cont.
19	Acoustic Droplet Ejection Compound Conservation Reutilize Source plates Storage time-dependent on method so yet unrealized Obviates the need for online store In house developments or possibly Tekcel Potentiates Closed-Loop UHTS
20	Where Do We Need Solutions? Source/Storage Plates Development Speed of Echo Compound Cherry Picking Online CRC dilution-Picoraptr? Automated Storage and Retrieval Automated Sealing and Unsealing Plates Controlled Environment-Compound Storage? Increased Flexibility in Scheduling Must Be Seamless with Compound Management-1536 source filling and tracking
21	Definitive Impact UHTS capability Cost advantage due to miniaturization, consumables and waste management Auditing feedback Compound conservation Hit picking capability Decreased systematic error-↑Accuracy & Precision **Sweeping changes to current compound supply line (compounds are reformatted at the time of the assay vs. pre-plating or aqueous dilution.)
22	Future Endeavors Implementing more Echo 550s Co-developing an optimum source plate Online storage and retrieval Closed loop screening
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24	Acknowledgements Bristol-Myers Squibb Martyn Banks Alastair Binnie Jonathan O’Connell Yvonne Fitzgerald Jim Myslik Mark Gilchrist Kim Marmora Jennifer Zewinski Normand Cloutier Ben Carvalho Joe Yacobucci Dan Maloney Neil Burford David Harden Angela Cacace Tatyana Zvyaga Jason Ansaldo Moneesh Chatterjee Sandra Matson