Notes
Slide Show
Outline
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Integration of Sample Management & HTS
  • Michael Longden, John Morin, Dominick Mobilio
    Wyeth Research, Pearl River, NY
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Why Automate Compound Management?
  • Anticipated Benefits


    • Speed of adding new compounds to the library
    • Hit-picking: the weakest link in HTS
      • Increase speed
      • Reduce necessity to access more samples than necessary
    • Speed and organization of automated plate storage
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Why Automate Compound Management?
  • Anticipated Benefits


    • Speed of adding new compounds to the library
    • Hit-picking: the weakest link in HTS
      • Increase speed
      • Reduce necessity to access more samples than necessary
    • Speed and organization of automated plate storage
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Advent Compound Dissolution & Plating Robot
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Input: compound powders
Output: DMSO solutions in plates and/or microtubes
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60,000 Compounds dissolved & plated per month
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Why Automate Compound Management?
  • Anticipated Benefits


    • Speed of adding new compounds to the library
    • Hit-picking: the weakest link in HTS
      • Increase speed
      • Reduce necessity to access more samples than necessary
    • Speed and organization of automated plate storage
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Decision: 2000
  • Massive systems seem inflexible and vulnerable
  • Not happy with heat sealed plates & piercing
  • Unenthusiastic about hit-picking options
  • TTP introduces modular comPOUND store in 2000
  • Brilliant new application of mature technology
  • Microtubes stored cold & inert with auto-decapping
  • TekCel & TTP agree to collaborate on hybrid system
  • Modularity reduces scale & initial cost of system
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Results
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Diagrammatic Overview
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Inside the TTP comPOUND Store
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TTP/TekCel Installation: 2003
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Loading in Parallel
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TTP comPILER Overview
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Sample Transfer from Tube to Plate in TekCel TekBench
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Automatic Transfer of New Plates to Frozen Storage
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One Man, with Random Access to 500,000 Tubes
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Factors Affecting Throughput
  • Number of comPOUND units
  • Odd or even number of comPOUND units
    • Software fix possible
  • Number of tubes per job
  • Number of jobs per day
    • Software fix to be released
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Tubes to Plates
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Compound Stability
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Water uptake in DMSO by Karl Fischer titration
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Picking the same 1,849 hits again and again
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Why Automate Compound Management?
  • Anticipated Benefits


    • Speed of adding new compounds to the library
    • Hit-picking: the weakest link in HTS
      • Increase speed
      • Reduce necessity to access more samples than necessary
    • Speed and organization of automated plate storage
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TekCel SealTite Lid: 1999-2003
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TekCel Modular Cluster
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Automated Random Access Plate Retrieval
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Automated Plate Processing
  • 100 plates from each cluster per day
  • Nov. 7th 2003 to January 7th 2004
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IT Infrastructure
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The End of the Beginning
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Acknowledgments
  • Wyeth Research: Jeff Paslay, Jim LaRocque, Craig Lamison, Adam Pitkin, Ed Bennett, Warren Dougherty, Paul Twesten, Susan Tomczyk, Zenon Bachir, Beverly Eccles, Guy Carter, Marc Tischler, Alvin Bach, Donald Herold, Jennifer Broderick, Heather Frisbie
  • TekCel:  Andy Zaayenga, Julian Warhurst, Peter Niggemann, Steve Fillers, Ed Kay, Alex King, Stuart Naylor
  • TTP:  Philip Blenkinsop, Jas Sanghera, Simon Tullett, Russell Hind, Noel Benn, Alex Reid, James Gibson
  • Advent Design Corp: Bill Chesterson, Jim Patton